Cyclophosphamide exerts an anti-metastatic effect by reducing the expression of MMP-2 and -9 in Saos2 osteosarcoma cells

Purpose: Although osteosarcoma is the most common primary malignant bone tumor among adolescents and young adults, molecular mechanisms underlying development of cancer are not fully elucidated. Cyclophosphamide (CYC) an alkylating antineoplastic agent widely used in treatment regimens various cancers chronic inflammatory diseases. The aim this study to reveal effect CYC on Saos-2 cells at level by determining expression levels some matrix metalloprotease proteins (MMP-2 MMP-9) progression related genes.
 Materials Methods: Cytotoxicity analyzes were performed applying developed human (Saos-2) cell cultures. determined dose was applied 2D lines for 12, 24 48 hours. As a result application, MMPs, which metastatic markers these cultures, immunocytochemical method.
 Results: While MMP-2 MMP-9 protein increased control (PBS) groups correlation with extend duration cultures created; decreased administration.
 Conclusion: It that genes play role metastasis such as application cells. These results shed light studies cancer.